TECHNICAL SKILLS:

Softwares used: MS word, WordPerfect, Microsoft Excel, Microsoft Power Point, Adobe PhotoShop, Acrobat reader, Chroma, Clone Manager, Mac DNAsis, MacDraw, MacWrite, Chem draw, Plasmid Artist, End Note, NIH Image, Persuasion, MS Office, MS Windows '95, '98 & 2000, MS Windows NT, Molecular Analyst, PeakSimple, MicroSeq. Microarray Suite 5.0 ( Confidential, Inc. ), Expression Array Manager ( Confidential, Microbiology, UW), Spot - on ( Confidential, Microbiology, UW), Image QuaNT, Confidential ( Confidential Confidential, Inc. ), Decision site for Functional Genomics ( Confidential, Inc. ), Sequence Analyzer.

PROFESSIONAL EXPERIENCE:

Confidential, Buffalo, NY

Confidential

Responsibilities:

• As a Confidential in Confidential, I am working on one of the ribosomal small subunit proteins (S15) to analyze and understand the evolutionary pathways in the living organisms leading to identifying Confidential, the oldest living species from the bacterial phylum from which all living organisms descended. Since ribosomes are the most conserved macromolecular assemblies consisting of ribonucleoproteins responsible for protein synthesis in all organisms, the phylogenetic analysis of its components is the best choice for the study of deep evolutionary transitions.

Confidential, Buffalo, NY

Lab Manager -Temporary position

Responsibilities:

• As a Lab Manager at Confidential, my tasks were to supervise day-to-day activities of the Lab, manage the resources, provide over all direction to the lab in line with company’s goals, write SOPs, validate the procedures & instruments, establish and strengthen cGMP & QC procedures, perform QC function {for DNA isolation from BAC clones, fluorescence probe production used in FISH (fluorescence in situ chybridization) and chromogenic probe production used in CISH (chromogenic in situ hybridization)}, perform troubleshooting and train other members of the team.

Confidential, Lake Forest Park, WA

Director Microbial ID Lab

Responsibilities:

• Supervised day-to-day activities of the Microbial ID Lab. Wrote SOPs, validation SOPs and technical reports, carried out validations studies, wrote validation reports, implemented cGMP requirements and as a result passed all the audits. I also took care of 16s rDNA sequence database, performed QC function for over five years and generated high quality and accurate microbial ID reports for pharmaceutical and food companies.

Confidential, Seattle, WA

Research Manager

Responsibilities:

• As Research Manager at Confidential, I provided leadership to molecular biology section and help establish procedures for microbial DNA isolation, sequencing, sequence data analysis and Pulsed Field Gel Electrophoresis (PFGE). I also worked as a liaison between Confidential and Biomarieux to validate Vitek2 instrument for microbial identification. To keep myself current with the new developments in the field, I under took in Confidential
• I also established a new sequencing facility for microbial identification (similar to the one at MEI) and provided consultation and to the staff in Molecular Biology techniques on daily basis. I also participated in the grand rounds and helped set up Pulsed Field Gel Electrophoresis (PFGE) system for subtyping microorganisms.

Confidential, Seattle, WA

Research Coordinator/Consultant

Responsibilities:

• Confidential is a core service lab providing microarray analysis for various labs on UW campuses and external scientists for their bioinformatics projects. As Research Coordinator/Consultant, I liaised between the clients and technical staff at Confidential and helped them with microarray experimental design and trouble shooting. I also provided high throughput expression microarray data analysis using Microarray Suite 5.0 ( Confidential, Inc. ), Expression Array Manager (in-house developed software at Confidential, UW), Spot-on ( Confidential, Microbiology, UW), Image QuaNT, Confidential ( Confidential Confidential, Inc. ), Decision site for Functional Genomics ( Confidential, Inc. ). To keep myself current with the new developments in the field, I under took several bioinformatics courses (on-campus and off-campus including webinars). For some clients, I provided technical expertise, problem solving, and consultation to clients regarding microarray experimental design and data analysis using bioinformatics tools. Acting as one of the instructors for the microarray data analysis workshop, I also delivered lectures to diverse audience. In one unique situation with one of our clients, I analyzed the expression microarray data, prepared PowerPoint presentation and presented at a conference on their behalf.

Confidential, Seattle, WA

Research Analyst

Responsibilities:

• I worked on a bioremediation research project with a goal to clean up the soil and ground water table contaminated with polychlorinated chemicals using bioremediation approaches. It involved isolation of anaerobic bioremediation bacteria responsible for degradation of polychlorinated chemicals from contaminated soils, anaerobic growth, identification and characterization (using Gas Chromatography under anaerobic conditions).

Confidential, Bothell, WA

Research Associate III

Responsibilities:

• EDEN Bioscience is a pioneer plant biotech Company in the Puget Sound area. I was hired to perform Gene cloning, gene expression, protein isolation experiments and developing & testing new strategies. I helped develop a bacterial strain caring recombinant plasmid capable of high expression of recombinant protein (responsible for higher yields and antimicrobial properties) and maintaining itself in bacteria without any selective antibiotic gene.

Confidential, Seattle, WA

Postdoctoral Fellow

Responsibilities:

• Confidential is another plant biotech Company in the Puget Sound area. I was hired to perform gene cloning to generating transgenic Arabidopsis plants and their evaluation and characterization. It involved transformation of Arabidopsis, using Agrobacterium tumefacience binary plasmid system and tissue culture to develop transgenic Arabidopsis.

Confidential, Seattle, WA

Graduate Student

Responsibilities:

• To carry out protein engineering or domain switching between cry1Aa and cry1Ba, oligonucleotide primers were designed that would introduce unique restriction enzyme sites. Various E. coli strains expressing wild-type or recombinant Confidential genes were grown and the plasmid DNA or inclusion proteins were isolated and analyzed or manipulated by using standard molecular biology techniques. Using standard cloning procedures several hybrid gene consisting of several of domains from different Confidential strains were synthesized, cloned and oriented in such a way to allow transcription from the lac- promoter of the pUC type plasmid, were expressed maximally in E. coli. The Confidential protein inclusion bodies were isolated and analyzed by SDS-polyacrylamide gel electrophoresis & western blots and protein concentrations determined.
• Purified B. thuringiensis protein inclusion bodies, isolated from the recombinant E. coli clones, were tested using insect larvae bioassays (for toxicity to the larvae of the lepidopteran, Manduca sexta and the coleopteran, Chrysomela scripta) and bioanalytical methods (e.g. Western blot, SDS-PAGE, sample purity assays, total protein determination) and sought solutions to technical problems associated with the performance of insect bioassays employing transgenic proteins.
• All the genetic constructs were sequenced for all the cry genes. Each domain of each gene was sequenced from both directions to determine the sequence of the domains and the regions between them.
• Some of the Confidential genes were also transformed into tobacco plants using Agrobacterium tumefacience binary plasmid system under the control of either a constitutive promoter or wound-inducible promoter. The biological activity of transgenic proteins derived from genetically modified plants was tested for insecticidal activity using Manduca sexta larvae.
• I also was Teaching Assistant for various molecular biology, biology, genetics and botany courses for three years.

Confidential, Seattle, WA

Responsibilities:

• Using pant molecular biology and microbiology techniques, I generated transgenic plants by transformation of Tobacco and Poplar with various insecticidal Bacillus thuringiensis (Bt) genes. I also carried out insect feeding and survival assays on those Confidential transgenic Tobacco plants using Manduca sexta larvae. I was also involved in a project focused on cleaning up the soil and ground water table contaminated with polychlorinated chemicals using bioremediation approaches. It involved cloning and expression of bioremediation related bacterial genes (responsible for degradation of polychlorinated chemicals) in transgenic plants (Tobacco, Poplar etc) using Agrobacterium tumifaciens (a plant pathogen) and electroporation.