Quality Assurance RESUME NJ - Hire IT People

Skill Section

Stock culture preparation
Tissue culture
Sterility testing
Enzyme extraction and purification
Utilization of DNA probes
Organization of batch and simultaneous testing

Employment History
Quality Assurance
04/2012 – Present Confidential,Irvine, CA

Use of controlled documents for raw data collection and calculations
Aseptic techniques, use of class 100 hoods; clean rooms; and sterility suites
Use of Clostridium sporogenes and Bacillus subtilis as B.I.’s
Personnel, environmental, and device bioburdens
Determination of D and Z values
Growth promotion tests
Serial dilutions and enumerations
P.M. monitoring; clean room pass through
Microbial I.D. via morphology, gram stains, and Vitek 2
LIMS system for data entry
Closed container integrity validations
FDA and cGMP work enviroment

Private Tutor
04/2010 – 04/2012 Confidential,

Biochemistry, Math, Microbiology, Biology, customized tutorial programs for college students, MCAT, ASVAB, and CBEST preparation

Microbiology Technician

11/1997 - 03/2010

Confidential,

Hesperia, CA

Gram stains ,wet mounts, blood culture collection, scoth tape specimen collection.API test strip inoculation and readings, RPR\'S, ova and parasite specimen collection, catalase tests, phlebotomy, histories, physicals, minor to moderate surgical procedures ,interpretation of diagnostic tests developed and implemented cost saving protocols, public speaking for the purpose of patient recruitment, supervision of clinical personnel.

Laboratory Assistant/Phlebotomist

08/1994 - 08/1995

Confidential,

Victorville, CA

In-patient and Out-patient phlebotomy, plating C. difficil tests, CBC\'s, PT & PTT\'s, CHEM7 and Chem 20 chemistry panels, gram stains ,temperature logs, machine calibrations.

Microbiology Technician

03/1992 - 06/1994

Confidential,

Santa Fe Springs, CA

Specimen assession and triage, culture and sensitivities, O&P specimen processing to be read by a technologist, gram and acid fast stains, blood culture incubation, DNA probes for GC/Chlamydiae, API test strip inoculation and reading, data entry, equiptment maintenance and calibration, temperature logs, wet mounts, KOH preps, electronic viable counts, broth and slant media inoculations, file MSDSworksheets, bench top disinfection, autoclave culture plates before disposal.

Biochemistry Research Assistant

03/1992 - 06/1994

Confidential,

Los Angeles, CA

Research(literature, computer, archives),inventory logs, wash glassware, calibrate equiptment/instruments, assist with enzyme extraction of "crassula argentia" and subsequent purification, plan/organize and conduct parallel kinetic studies by varying subtrates/co-enzymes, and temperatures. Present findings to research group and before presentation committees.

Research Assistant

06/1990 - 03/1992

Confidential,

Pasadena, CA

Hired initialing to provide microbial control of tissue cultures. Expanded into inoculation and maintenance of tissue cultures (tumor, glial, squamous, lymphocyte cells).Cryogenic freezing of multiple generations of cell lines. Thawing cryogenic cell lines and doing viability counts. Radioactive tagging of tumor cells for tracing in cell flow cytometry.Operated steri-cell for separation of lymphocytes from whole blood. Theory was to infuse patients with autologous activated T-lymphocytes to fight tumor cells in body. Principal investigator also wanted to know the maximum number of generations of a cell line could be allowed to artificially reproduce before DNA errors present. I conducted assays independently measuring rate of substrate utilization as the temperature, pH, salinity, and concentrations were varied. In this position I first became familiar with using liquid nitrogen and laminar flow hoods.

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