SUMMARY:

• Bioinformatics Analyst with ten plus years of diverse work history in the biomedical/biotechnology field.
• Possess extensive experience in Biomedical laboratories and Bioinformatic data analytics.
• Interested in career development as a data science professional.
• Navigating file - systems and executing commands in an UNIX environment.
• Familiarity with several common scripting and relational database languages i.e. Perl, R, Java, SQL, Shell
• Operating and troubleshooting Perl & Shell Data Processing Pipelines via Command Line.
• Running applications remotely on data servers including cloud based servers e.g. AWS
• Extensive Experience in MS Office Applications including MS Excel data analysis/management and MS PowerPoint presentation creation.
• Utilizing Project Management tools e.g. Asana, Instagantt, Jira
• Usage of Cloud Based Data Storage Tools e.g. Dropbox, GoogleDrive
• Software/SAAS User Manual & SOP creation and development
• Analysis and Application of Complex and Large Datasets e.g. Next Generation Sequencing Data
• Automation experience and programming with scientific robotic platforms such Beckman Biomek, Qiagen Qiagility & Qiacube, Roche MagNA Pure
• User end familiarity with several common bioinformatic software tools including BLAST, Confidential Genomics Workbench, Geneious, Oligo, Primer 3, Sequencher, etc.
• Ability to search, review, & interpret advanced scientific literature.
• Possess experience working in Linux, Mac, & Windows OS

PROFESSIONAL EXPERIENCE:

Bioinformatics Analyst

Confidential, Rockville, MD

Responsibilities:

• Performed primary analysis of large biological datasets including gene & protein annotation for submission to public government database.
• Operated and troubleshot internal Perl Language Annotation and Submission data pipelines in a UNIX command line environment.
• Edit, Transform and Upload Metadata SQL database via Perl Scripts.
• Functioned as liaison between internal organization data processing team and external clients to ensure data QA/QC
• Manually annotate, curate, & review client submitted data in order to ensure adherence to organization data format standards
• Utilize R for data analysis of large datasets and to create visualizations.
• Utilize Jira to update and track completion project requirements and milestones.
• Create new and update existing technical SOPs on Internal Wiki

Bioinformatics Analyst

Confidential, Rockville, MD

Responsibilities:

• Primary analysis of Confidential algorithmic output, including exporting, formatting, interpretation, and comparison of metagenomic & microbiome data.
• Participate in Beta Testing of new Confidential proprietary metagenomic analytical software including creation of in silico training sets.
• Execute Shell and Perl Data Processing Scripts via Command Line in UNIX environments.
• Logging into remotely and running applications on both on site & cloud based servers e.g AWS
• Modifying R scripts for data analysis of Excel spreadsheets.
• Utilize source code management tools i.e. Github
• Orthogonal evaluation of algorithmic results with outside bioinformatics tools e.g. Confidential Genomics Workbench, BLAST, etc.
• Identity and develop improvements to data processing pipeline in conjunction with other team members through tools i.e. Jira
• Contribute written material to scientific and technical literature and reports as needed.
• Develop and revise SOPS on internal Wiki.

R&D Next-Gen Sequencing Specialist

Confidential, College Park, MD

Responsibilities:

• Accession and track incoming laboratory samples using LIMS software
• Prepare genomic libraries of bacterial isolate and metagenomic samples for whole genome shotgun sequencing
• Identify and implement new methods and products for improvement and optimization of DNA Extraction and library construction
• Operate, monitor, and troubleshoot Illumina GAII NGS platform.
• Utilize Agilent Bioanalyzer and ABI ViiA7 RT-PCR to quantify and QC/QA genomic library preps.
• Contribute to writing peer reviewed scientific literature.

R&D Technical Specialist/Technical Specialist

Confidential, Gaithersburg, MD

Responsibilities:

• Developed and balanced complex genetic disease panels for clinical applications on Illumina Confidential Sequencing GA and High Seq. platforms.
• Prepared gene panel libraries for Illumina Sequencing with both PCR Pooling and Raindance protocols.
• Functioned as a team leader in the company’s Capillary Sequencing unit. Oversaw maintenance, operation, and validation of ABI 3730 DNA Analyzers to ensure generation of high quality sequence data.
• Monitored a complex clinical data pipeline at multiple levels.
• Trained subordinate team members to follow applicable clinical SOPS in order to ensure accurate sequencing results.
• Directed team adherence to relevant industrial regulations i.e. Confidential & Confidential in order acquire and maintain licensing status.
• Performed primary analysis of ABI generated DNA sequences for the purposes of adequate exon coverage and mutation identification.

Laboratory Technician

Confidential, Ithaca, NY

Responsibilities:

• Utilized tissue culture techniques including propagation and transfection of mammalian cell lines.
• Designed and constructed various siRNA knockout, mammalian transfection and expression vectors.
• Harvested and purified protein from cells for analysis.
• Isolated RNA from mammalian tissues and cells for later reverse transcription
• Prepared media and samples for daily laboratory operation.
• Coordinated compliance with radioactive and chemical safety regulations.