Genomics Laboratory Scientist Resume Atlanta, GA - Hire IT People

SUMMARY:

Ph.D in Molecular Biology with solid knowledge in genomics, microbiology, biotechnology, laboratory testing and public health.
Expertise in Next Generation Sequencing with end - to-end workflow experience by sequencing thousands of samples on Illumina MiSeq and HiSeq, experience on PacBio and knowledge on Nanopore.
Extensive experience with Confidential data analysis, such as whole genome assembly, genetic variation detection and phylogenetic analysis using CLC Genomics Workbench, Geneious and PacBio SMRT Portal.
Extensive experience with developing and validating Multiplex, Real-Time, Fluorogenic Confidential (TaqMan) Assay to identify clinical isolates of public health importance.
15 years extensive experience on diverse molecular techniques.
Experience on microscopy, fluorescence microscopy and confocal laser scanning microscopy.
Ability to work both independently and as a team member; good scientific communication skills including organization, presentation, manuscript and proposal preparation; extremely organized and attentive to details; self-motivated and passionate with research.

MAIN SCIENTIFIC SKILLS:

Molecular Biology and Confidential: DNA isolation, RNA isolation, Protein extraction; Primer and Probe design, Confidential, Realtime Confidential, Multiplex Fluorogenic Confidential, Library construction for Confidential, QC, design and perform Illumina MiSeq and HiSeq sequencing, Sanger sequencing.

Microbiology: Identify, culture and transformation of various microbe strains; DNA and RNA isolation from microbe samples.

Bioinformatics: Proficiency in DNA sequence analysis, alignment and phylogenetic analysis via software (e.g. BioEidt, MEGA, Mauve, Muscle, Clustal and Blast n/p/x, Analysis of structural and functional characterization of protein using software (e.g. Swiss-model). Whole genome assembly, Genetic variations detection via CLC Genomics Workbench and DNASTAR NGen.

RESEARCH AND WORK EXPERIENCE:

Genomics Laboratory Scientist

Confidential, Atlanta, GA

Responsibilities:

Use of Comparative Genomics to Improve Species-level Leishmania Diagnostic Testing
Performing culture to propagate Leishmania parasites and extracting genomic DNA from harvested cultures.
Producing libraries for parallel/next generation sequencing analysis from genomic DNA preparations.
Sequencing on Sanger, Illumina and PacBio platforms.
Performing comparative genomics to identify variable regions within the Leishmania genomes that can be used in species/strain-specific assays, which may be associated with particular clinical phenotypes and suboptimal therapeutic responses.
Performing amplicon sequencing for multi-locus sequencing typing (MLST) targeting enzymes identified from comparative genomics data.
Developing and validating multiplex TaqMan realtime Confidential assays capable of simultaneous identification of multiple species in the same reaction.
Advancing Detection of Cyclosporiasis (C. cayetanensis) Using Genomics-based Laboratory Surveillance and Reference Diagnostics
Performing purification of Cyclospora cayetanensis oocysts through discontinuous sucrose and cesium chloride gradients.
High molecular weight genomic DNA extraction from oocysts purified from clinical specimens.
High quality whole genome library construction from low input DNA.
Whole genome sequence assembly and phylogenetic relationship analysis through mitochondrial genome.
Analyze data using various software platforms (CLC Genomics Workbench; Geneious; PacBio SMRT Portal; OpGen MapSolver) to determine results, prepare for presentations and publications.
Actively participate in the laboratory quality management system by ensuring the full range of applicable quality controls are correctly conducted; Assist in proper collection and storage of laboratory data and samples including keeping laboratory inventories up to date.

Laboratory Technologist

Confidential, Norcross, GA

Responsibilities:

DNA and RNA extraction from various specimen; Genotyping of various samples via Confidential and Quantitative Confidential ; Library construction and QC for Confidential ; Conduct 16S and 18S rRNA sequencing end-to-end workflow; Whole genome sequencing; RNA sequencing (total RNA-Seq and mRNA-Seq); Perform sequencing on Illumina MiSeq and HiSeq platform.

Postdoctoral Research Associate

Confidential, Knoxville, TN

Responsibilities:

Cloning, expression analysis and functional characterization of biomass related genes using genomic, genetic and biotechnology approaches.

Postdoctoral Research Associate

Confidential, Ames, IA

Responsibilities:

Isolation, identification, and physiological function characterization of triacylglycerol for the purpose of biofuel
Environment stresses tolerance research via transgenic Arabidopsis

Research Assistant

Confidential

Responsibilities:

Hormone-autotrophic cell culture and specific expression genes in Confidential ginseng for biotechnology medicine.
Systemic defense responses induced by microbe and insects.

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Genomics Laboratory Scientist Resume

Atlanta, GA

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